Nanoblade delivery and incorporation of quantum dot conjugates into tubulin networks in live cells

Nano Lett. 2012 Nov 14;12(11):5669-72. doi: 10.1021/nl302821g. Epub 2012 Nov 5.

Abstract

Quantum dots (QDs) have not been used to label cytoskeleton structure of live cells owing to limitations in delivery strategies, and QDs conjugation methods and issues with nonspecific binding. We conjugated tubulin to QDs and applied the emerging method of photothermal nanoblade to deliver QD-tubulin conjugates into live Hela cells. This method will open new opportunities for cytosolic targeting of QDs in live cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cytoskeleton / metabolism
  • Cytosol / metabolism
  • Drug Delivery Systems
  • Endosomes / chemistry
  • HeLa Cells
  • Humans
  • Light
  • Microscopy / methods
  • Nanotechnology / methods*
  • Photochemistry / methods
  • Quantum Dots*
  • Tubulin / chemistry*

Substances

  • Tubulin