Thyroid autoantibody measurement by enzyme immunoassay

Indian J Clin Biochem. 1997 Dec;12(1):35-9. doi: 10.1007/BF02867952.

Abstract

Thyroid antibodies are commonly utilized in the assessment and diagnosis of autoimmune thyroid disorders. We compared the measurements of antithyroglobulin and antithyroidperoxidase antibodies by enzyme immunoassay with that of the conventional agglutination method. This fully automated enzyme immunoassay is more specific and cost effective than the agglutination method. Further this is a very quantitative and rapid method producing results in two hours as compared to at least twenty=four hours required by the conventional method. Antithyroidperoxidase antibodies determined by enzyme immunoassay are more specific and sensitive in the diagnosis of Hashimoto's thyroiditis than the antithyroglobulin antibodies.

Keywords: EIA; antithyroglobulin antibodies; antithyroidperoxidase antibodies.