SILAC-based quantitative proteomic analysis of gastric cancer secretome

Proteomics Clin Appl. 2013 Jun;7(5-6):355-66. doi: 10.1002/prca.201200069. Epub 2013 May 21.

Abstract

Purpose: Gastric cancer is a commonly occurring cancer in Asia and one of the leading causes of cancer deaths. However, there is no reliable blood-based screening test for this cancer. Identifying proteins secreted from tumor cells could lead to the discovery of clinically useful biomarkers for early detection of gastric cancer.

Experimental design: A SILAC-based quantitative proteomic approach was employed to identify secreted proteins that were differentially expressed between neoplastic and non-neoplastic gastric epithelial cells. Proteins from the secretome were subjected to SDS-PAGE and SCX-based fractionation, followed by mass spectrometric analysis on an LTQ-Orbitrap Velos mass spectrometer. Immunohistochemical labeling was employed to validate a subset of candidates using tissue microarrays.

Results: We identified 2205 proteins in the gastric cancer secretome of which 263 proteins were overexpressed greater than fourfold in gastric cancer-derived cell lines as compared to non-neoplastic gastric epithelial cells. Three candidate proteins, proprotein convertase subtilisin/kexin type 9 (PCSK9), lectin mannose binding 2 (LMAN2), and PDGFA-associated protein 1 (PDAP1) were validated by immunohistochemical labeling.

Conclusions and clinical relevance: We report here the largest cancer secretome described to date. The novel biomarkers identified in the current study are excellent candidates for further testing as early detection biomarkers for gastric adenocarcinoma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Amino Acids / chemistry
  • Amino Acids / metabolism*
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Chromatography, High Pressure Liquid
  • Computational Biology
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins / chemistry
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Isotope Labeling
  • Mannose-Binding Lectins / chemistry
  • Mannose-Binding Lectins / metabolism
  • Mass Spectrometry
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / metabolism
  • Proprotein Convertase 9
  • Proprotein Convertases / chemistry
  • Proprotein Convertases / metabolism
  • Proteins / chemistry
  • Proteins / metabolism*
  • Proteomics*
  • Serine Endopeptidases / chemistry
  • Serine Endopeptidases / metabolism
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology

Substances

  • Amino Acids
  • Biomarkers, Tumor
  • Intercellular Signaling Peptides and Proteins
  • LMAN2 protein, human
  • Mannose-Binding Lectins
  • Membrane Transport Proteins
  • PDAP1 protein, human
  • Proteins
  • PCSK9 protein, human
  • Proprotein Convertase 9
  • Proprotein Convertases
  • Serine Endopeptidases