Pre- and postnatal development in the cynomolgus monkey following administration of ABT-874, a human anti-IL-12/23p40 monoclonal antibody

Birth Defects Res B Dev Reprod Toxicol. 2012 Dec;95(6):431-43. doi: 10.1002/bdrb.21034. Epub 2012 Dec 4.

Abstract

Background: ABT-874 is an anti-IL-12/23 monoclonal antibody that binds to the p40 subunit of human IL-12 and IL-23. As part of its preclinical safety assessment, studies were conducted to assess its potential effects on pre- and postnatal development in cynomolgus monkeys.

Methods: In the embryo-fetal development studies, ABT-874 was administered once weekly subcutaneously to adult female cynomolgus monkeys at doses of 0, 5, 25, or 100 mg/kg during gestation days (GD) 20 to 48. Fetuses were examined for external, visceral, and skeletal development on GD 100 or 150. In the pre- and postnatal study, ABT-874 was administered once weekly subcutaneously to adult female cynomolgus monkeys at doses of 10, 50, or 200 mg/kg from GD 20 through postpartum day 182. Infants were examined from birth up to 9 months of age for morphological and functional development. Potential effects on the infant immune system were evaluated by immunophenotyping of peripheral blood lymphocytes and by T-dependent antibody response to KLH.

Results: There was no ABT-874-related maternal toxicity or adverse effects on fetuses or infants. ABT-874 was present in maternal and fetal serum at GD 100 and 150, and in infant serum through day 63 postbirth. ABT-874 was also present at low levels in breast milk through postpartum day 175.

Conclusions: Exposure of cynomolgus monkey fetuses and infants to ABT-874 had no adverse effects on embryo-fetal or postnatal development.

MeSH terms

  • Animals
  • Animals, Newborn
  • Antibodies, Monoclonal / toxicity*
  • Antibodies, Monoclonal, Humanized
  • Antibody Formation / drug effects
  • Body Weight / drug effects
  • Dose-Response Relationship, Drug
  • Embryo, Mammalian / drug effects*
  • Embryonic Development / drug effects*
  • Female
  • Fetal Development / drug effects*
  • Hemocyanins / pharmacology
  • Immune System / drug effects
  • Injections, Subcutaneous
  • Lactation / drug effects
  • Lymphocytes / drug effects
  • Macaca fascicularis / physiology*
  • Male
  • Maternal Exposure / adverse effects*
  • Pregnancy
  • Prenatal Exposure Delayed Effects / chemically induced
  • Toxicity Tests

Substances

  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Hemocyanins
  • briakinumab
  • keyhole-limpet hemocyanin