Analysis of porcine parvoviruses in tonsils and hearts from healthy pigs reveals high prevalence and genetic diversity in Germany

Arch Virol. 2013 Jun;158(6):1173-80. doi: 10.1007/s00705-013-1603-0. Epub 2013 Jan 22.

Abstract

In the present study, tonsils and hearts from 100 pigs were collected in a German slaughterhouse in 2010 and tested for porcine parvoviruses (PPV, PPV2, PPV3 and PPV4). PPV was observed in 60 of 100 hearts and 61 of 100 tonsils, and PPV2 was observed in 55 of 100 hearts and 78 of 100 tonsils. PPV3 and PPV4 were found in 20 and 7, respectively, of the 100 tonsils tested, but not in the heart samples. Positive samples of PPV, PPV2 and PPV3 were analyzed by nucleotide sequencing, and phylogenetic analysis revealed at least two distinct lineages for each virus in the German samples. The high detection rate of PPV, PPV2 and PPV3 in healthy animals and their genetic diversity highlights the importance of continuous monitoring of these viruses and their zoonotic potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Genetic Variation / genetics
  • Germany / epidemiology
  • Heart / virology*
  • Molecular Sequence Data
  • Palatine Tonsil / virology*
  • Parvoviridae Infections / epidemiology
  • Parvoviridae Infections / veterinary*
  • Parvoviridae Infections / virology
  • Parvovirus, Porcine / genetics*
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA / veterinary
  • Swine / virology
  • Swine Diseases / epidemiology
  • Swine Diseases / virology*

Associated data

  • GENBANK/KC296743
  • GENBANK/KC296744
  • GENBANK/KC296745
  • GENBANK/KC296746
  • GENBANK/KC296747
  • GENBANK/KC296748
  • GENBANK/KC296749
  • GENBANK/KC296750
  • GENBANK/KC296751
  • GENBANK/KC296752