Application of molecularly imprinted polymers to determine B1, B2, and B3 fumonisins in cereal products

J Sep Sci. 2013 Feb;36(3):578-84. doi: 10.1002/jssc.201200753.

Abstract

The aim of this study was to develop an analytical method for qualitative and quantitative determination of the B(1), B(2,) and B(3) fumonisins in cereal products. A LC coupled to an IT-MS was used as the analytical instrument. The AFFINIMIP FumoZON Molecularly Imprinted Polymer SPE cartridges (Polyintell) were used to isolate fumonisins from the analyzed samples and the clean-up step. Statistical parameters evaluated in some validation experiments were as follows: mean recovery 95-106%, precision <17% (expressed as recovery RSD). The developed method was used to determine fumonisins in 49 cereals (42 maize-based and seven wheat-based products). In most cases, concentrations of the studied compounds found in the analyzed samples were low. The highest total concentration of the B(1), B(2), and B(3) fumonisins was found in maize flour samples (range, 26-1102 μg/kg, mean 498 μg/kg).

Publication types

  • Evaluation Study

MeSH terms

  • Edible Grain / chemistry*
  • Food Contamination / analysis*
  • Fumonisins / chemistry
  • Fumonisins / isolation & purification*
  • Mass Spectrometry
  • Molecular Imprinting
  • Polymers / chemical synthesis
  • Polymers / chemistry*
  • Solid Phase Extraction / instrumentation
  • Solid Phase Extraction / methods*

Substances

  • Fumonisins
  • Polymers