Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections

J Clin Microbiol. 2013 Apr;51(4):1281-4. doi: 10.1128/JCM.00190-13. Epub 2013 Jan 30.

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Bacterial Infections / diagnosis
  • Bacterial Infections / microbiology
  • Coinfection / diagnosis*
  • Coinfection / microbiology*
  • Computational Biology / methods*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Sequence Analysis, DNA / methods*
  • Software