Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

Mol Biosyst. 2013 Apr 5;9(4):626-9. doi: 10.1039/c3mb25584k.

Abstract

Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / chemistry*
  • Detergents / chemistry
  • Membrane Proteins / chemistry*
  • Micelles
  • Octoxynol / chemistry*
  • Protein Stability
  • Solubility
  • Surface-Active Agents / chemistry

Substances

  • Carbohydrates
  • Detergents
  • Membrane Proteins
  • Micelles
  • Surface-Active Agents
  • Octoxynol