Lethal Crimean-Congo hemorrhagic fever virus infection in interferon α/β receptor knockout mice is associated with high viral loads, proinflammatory responses, and coagulopathy

J Infect Dis. 2013 Jun 15;207(12):1909-21. doi: 10.1093/infdis/jit061. Epub 2013 Feb 15.

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a widely distributed viral hemorrhagic fever characterized by rapid onset of flu-like symptoms often followed by hemorrhagic manifestations. CCHF virus (CCHFV), a bunyavirus in the Nairovirus genus, is capable of infecting a wide range of mammalian hosts in nature but so far only causes disease in humans. Recently, immunocompromised mice have been reported as CCHF disease models, but detailed characterization is lacking. Here, we closely followed infection and disease progression in CCHFV-infected interferon α/β receptor knockout (IFNAR(-/-)) mice and age-matched wild-type (WT) mice. WT mice quickly clear CCHFV without developing any disease signs. In contrast, CCHFV infected IFNAR(-/-) mice develop an acute fulminant disease with high viral loads leading to organ pathology (liver and lymphoid tissues), marked proinflammatory host responses, severe thrombocytopenia, coagulopathy, and death. Disease progression closely mimics hallmarks of human CCHF disease, making IFNAR(-/-) mice an excellent choice to assess medical countermeasures.

Keywords: CCHFV; coagulopathy; interferon α/β receptor knockout mice; pathology; proinflammatory response; thrombocytopenia.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Blood Coagulation Disorders / immunology
  • Blood Coagulation Disorders / pathology
  • Blood Coagulation Disorders / virology
  • Chemokines / metabolism
  • Cytokines / metabolism
  • Disease Models, Animal
  • Disease Progression
  • Hemorrhagic Fever Virus, Crimean-Congo / immunology*
  • Hemorrhagic Fever Virus, Crimean-Congo / physiology
  • Hemorrhagic Fever, Crimean / complications
  • Hemorrhagic Fever, Crimean / immunology*
  • Hemorrhagic Fever, Crimean / pathology
  • Humans
  • Liver / pathology
  • Liver / virology
  • Liver Failure, Acute / immunology*
  • Liver Failure, Acute / pathology
  • Liver Failure, Acute / virology
  • Lymphoid Tissue / pathology
  • Lymphoid Tissue / virology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptor, Interferon alpha-beta / genetics*
  • Receptor, Interferon alpha-beta / immunology
  • Spleen / pathology
  • Spleen / virology
  • Thrombocytopenia / immunology
  • Thrombocytopenia / pathology
  • Thrombocytopenia / virology
  • Viral Load
  • Viremia

Substances

  • Chemokines
  • Cytokines
  • Receptor, Interferon alpha-beta