Identification of nonvisual photomotor response cells in the vertebrate hindbrain

J Neurosci. 2013 Feb 27;33(9):3834-43. doi: 10.1523/JNEUROSCI.3689-12.2013.

Abstract

Nonvisual photosensation enables animals to sense light without sight. However, the cellular and molecular mechanisms of nonvisual photobehaviors are poorly understood, especially in vertebrate animals. Here, we describe the photomotor response (PMR), a robust and reproducible series of motor behaviors in zebrafish that is elicited by visual wavelengths of light but does not require the eyes, pineal gland, or other canonical deep-brain photoreceptive organs. Unlike the relatively slow effects of canonical nonvisual pathways, motor circuits are strongly and quickly (seconds) recruited during the PMR behavior. We find that the hindbrain is both necessary and sufficient to drive these behaviors. Using in vivo calcium imaging, we identify a discrete set of neurons within the hindbrain whose responses to light mirror the PMR behavior. Pharmacological inhibition of the visual cycle blocks PMR behaviors, suggesting that opsin-based photoreceptors control this behavior. These data represent the first known light-sensing circuit in the vertebrate hindbrain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Analysis of Variance
  • Animals
  • Biomechanical Phenomena
  • Biophysics
  • Calcium / metabolism
  • Embryo, Nonmammalian
  • Female
  • Male
  • Microscopy, Confocal
  • Morpholinos / pharmacology
  • Movement / drug effects
  • Movement / physiology*
  • Movement / radiation effects
  • Muscle Cells / drug effects
  • Muscle Cells / radiation effects
  • Neural Pathways / drug effects
  • Neural Pathways / physiology
  • Neural Pathways / radiation effects
  • Opsins / chemistry
  • Opsins / metabolism*
  • Photic Stimulation
  • Photoreceptor Cells, Vertebrate / drug effects
  • Photoreceptor Cells, Vertebrate / physiology*
  • Photoreceptor Cells, Vertebrate / radiation effects
  • Rhombencephalon / cytology*
  • Rhombencephalon / physiology
  • Stereotyped Behavior / drug effects
  • Stereotyped Behavior / physiology*
  • Stereotyped Behavior / radiation effects
  • Time Factors
  • Zebrafish

Substances

  • Morpholinos
  • Opsins
  • Calcium