IL-6 production by dendritic cells is dispensable for CD8+ memory T-cell generation

Biomed Res Int. 2013:2013:126189. doi: 10.1155/2013/126189. Epub 2012 Dec 30.

Abstract

Following activation, naïve CD8(+) T cells will differentiate into effectors that differ in their ability to survive: some will persist as memory cells while the majority will die by apoptosis. Signals given by antigen-presenting cells (APCs) at the time of priming modulate this differential outcome. We have recently shown that, in opposition to dendritic cell (DC), CD40-activated B-(CD40-B) cell vaccination fails to efficiently produce CD8(+) memory T cells. Understanding why CD40-B-cell vaccination does not lead to the generation of functional long-lived memory cells is essential to define the signals that should be provided to naïve T cells by APCs. Here we show that CD40-B cells produce very low amount of IL-6 when compared to DCs. However, supplementation with IL-6 during CD40-B-cell vaccination did not improve memory generation. Furthermore, IL-6-deficient DCs maintained the capacity to promote the formation of functional CD8(+) effectors and memory cells. Our results suggest that in APC vaccination models, IL-6 provided by the APCs is dispensable for proper CD8(+) T-cell memory generation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer*
  • Animals
  • B-Lymphocytes / immunology
  • B-Lymphocytes / transplantation
  • CD40 Antigens / genetics
  • CD40 Antigens / immunology
  • CD8-Positive T-Lymphocytes / immunology*
  • Dendritic Cells / immunology*
  • Dendritic Cells / transplantation
  • Immunologic Memory*
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology*
  • Mice
  • Mice, Knockout

Substances

  • CD40 Antigens
  • Interleukin-6