399 tick specimens were collected from the body surface of police dogs in Chongqing municipality, provinces of Fujian, Guangdong, Hainan, Guangxi, Hebei, Henan, Shanxi, Jiangsu and Zhejiang. Nested-PCR and sequence testing were taken to investigate the prevalence of Babesia sp. in ticks. The results showed that Babesia vogeli was found in ticks infested on the body surface of police dogs, with a positive rate of 5.3%. The prevalence in Chongqing, Guangdong, Guangxi, Hainan and Zhejiang was 4/16, 3.6% (1/28), 12.5%(11/88), 3.3% (4/121) and 1/15, respectively. It suggested that there was a certain rate of infected ticks infested on the body suriface of police dogs, which contributed to the potential threat to staff. The prevention and control measures should be strengthened.