The effect of mesenchymal stromal cells on doxorubicin-induced nephropathy in rats

Cytotherapy. 2013 Jun;15(6):703-11. doi: 10.1016/j.jcyt.2013.02.002. Epub 2013 Mar 14.

Abstract

Background aims: The potential protective effects of mesenchymal stromal cells (MSCs) on some kidney diseases has been reported. However, the effect of MSCs on doxorubicin-induced nephropathy is still poorly understood.

Methods: Rats with doxorubicin-induced kidney injuries were treated with human cord-derived MSCs. Human MSCs were first labeled with 5-bromo-2'-deoxyuridine to track their homing in kidneys after infusion.

Results: Alleviation of proteinuria, decreased serum albumin, alleviation of lipid disorders and histologic alterations were found in rats 4 weeks after treatment with MSCs, particularly in rats that were given repeat doses. Decreases in serum levels of interleukin-6, tumor necrosis factor-α and prostaglandin E2 and decreases in messenger RNA levels of kidney tissue cylooxygenase-2 and EP4 were found in MSC-treated rats. MSC-treated rats also displayed an increase in serum interleukin-10 levels.

Conclusions: These results indicate that MSCs ameliorate doxorubicin-induced kidney injuries and inflammation, suggesting a potential clinical treatment for inflammatory kidney diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell- and Tissue-Based Therapy*
  • Cyclooxygenase 2 / blood
  • Dinoprostone / blood
  • Doxorubicin / toxicity
  • Gene Expression
  • Humans
  • Inflammation / pathology
  • Inflammation / therapy
  • Interleukin-6 / blood
  • Kidney Diseases / blood
  • Kidney Diseases / chemically induced
  • Kidney Diseases / pathology
  • Kidney Diseases / therapy*
  • Mesenchymal Stem Cell Transplantation* / adverse effects
  • Mesenchymal Stem Cell Transplantation* / methods
  • Mesenchymal Stem Cells / cytology*
  • Rats
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Doxorubicin
  • Cyclooxygenase 2
  • Dinoprostone