Increased muscle force production and bone mineral density in ActRIIB-Fc-treated mature rodents

J Gerontol A Biol Sci Med Sci. 2013 Oct;68(10):1181-92. doi: 10.1093/gerona/glt030. Epub 2013 Mar 22.

Abstract

Myostatin is a highly conserved member of the transforming growth factor-β ligand family known to regulate muscle growth via activation of activin receptors. A fusion protein consisting of the extracellular ligand-binding domain of activin type IIB receptor with the Fc portion of human immunoglobulin G (ActRIIB-Fc) was used to inhibit signaling through this pathway. Here, we study the effects of this fusion protein in adult, 18-month-old, and orchidectomized mice. Significant muscle growth and enhanced muscle function were observed in adult mice treated for 3 days with ActRIIB-Fc. The ActRIIB-Fc-treated mice had enhanced fast fatigable muscle function, with only minor enhancement of fatigue-resistant fiber function. The ActRIIB-Fc-treated 18-month-old mice and orchidectomized mice showed significantly improved muscle function. Treatment with ActRIIB-Fc also increased bone mineral density and serum levels of a marker of bone formation. These observations highlight the potential of targeting ActRIIB receptor to treat age-related and hypogonadism-associated musculoskeletal degeneration.

Keywords: Activin receptor type IIB; Muscle; Myostatin; Sarcopenia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activin Receptors, Type II / metabolism
  • Activin Receptors, Type II / pharmacology*
  • Aging / drug effects*
  • Aging / physiology*
  • Animals
  • Biomarkers / blood
  • Bone Density / drug effects*
  • Bone Density / physiology
  • Cell Line
  • HEK293 Cells
  • Humans
  • Immunoglobulin Fc Fragments / metabolism
  • Immunoglobulin Fc Fragments / pharmacology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muscle Contraction / drug effects*
  • Muscle Contraction / physiology
  • Muscle Strength / drug effects
  • Muscle Strength / physiology
  • Myostatin / metabolism
  • Orchiectomy
  • Peptide Fragments / blood
  • Procollagen / blood
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / pharmacology
  • Sarcopenia / drug therapy
  • Sarcopenia / pathology
  • Sarcopenia / physiopathology

Substances

  • Biomarkers
  • Immunoglobulin Fc Fragments
  • Mstn protein, mouse
  • Myostatin
  • Peptide Fragments
  • Procollagen
  • Recombinant Fusion Proteins
  • procollagen Type I N-terminal peptide
  • Activin Receptors, Type II
  • activin receptor type II-B