High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing

Adv Mater. 2013 Jun 4;25(21):2897-902. doi: 10.1002/adma.201205237. Epub 2013 Mar 26.

Abstract

Handpick single cancer cells: a modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.

Keywords: circulating tumor cell; electrospun nanomaterials; microfluidics; prostate cancer; whole exome sequencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Line, Tumor
  • Cell Separation / methods*
  • Exome / genetics*
  • Genomics
  • Humans
  • Lactic Acid / chemistry
  • Male
  • Microchip Analytical Procedures / methods*
  • Nanofibers*
  • Neoplastic Cells, Circulating / metabolism
  • Neoplastic Cells, Circulating / pathology*
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / pathology*
  • Sequence Analysis, DNA*

Substances

  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid