Molecularly imprinted SPE combined with dispersive liquid-liquid microextraction for selective analysis of telmisartan in biological and formulation samples

Bioanalysis. 2013 Apr;5(7):847-58. doi: 10.4155/bio.13.45.

Abstract

Background: The present communication describes the combination of molecularly imprinted SPE and dispersive liquid-liquid microextraction for the selective preconcentration and determination of telmisartan (TEL) in rat urine, plasma and pharmaceutical formulation by HPLC.

Results: Various factors that can affect the extraction efficiency of molecularly imprinted SPE and dispersive liquid-liquid microextraction were optimized. The LOD and LOQ were found to be 0.19 and 0.63 µg ml(-1) in urine, while in plasma it was found to be 0.28 and 0.87 µg ml(-1), respectively. The percentage recovery of TEL in different matrices was found to be in the range of 81-97%.

Conclusion: The proposed method may find wide applications in clinical, toxicological and QC laboratories for the routine analysis of TEL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II Type 1 Receptor Blockers / analysis*
  • Animals
  • Benzimidazoles / analysis*
  • Benzoates / analysis*
  • Liquid Phase Microextraction / methods*
  • Male
  • Molecular Imprinting / methods*
  • Rats
  • Rats, Wistar
  • Solid Phase Extraction / methods*
  • Telmisartan

Substances

  • Angiotensin II Type 1 Receptor Blockers
  • Benzimidazoles
  • Benzoates
  • Telmisartan