C333H ameliorated insulin resistance through selectively modulating peroxisome proliferator-activated receptor γ in brown adipose tissue of db/db mice

Biol Pharm Bull. 2013;36(6):980-7. doi: 10.1248/bpb.b13-00008. Epub 2013 Apr 6.

Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) is a unique target for insulin sensitizer agents. These drugs have been used for the clinical treatment of type 2 diabetes for almost twenty years. However, serious safety issues are associated with the PPARγ agonist thiazolidinediones (TZDs). Selective PPARγ modulators (SPPARMs) which retain insulin sensitization without TZDs-like side effects are emerging as a promising new generation of insulin sensitizers. C333H is a novel structure compound synthesized by our laboratory. In diabetic rodent models, C333H has insulin-sensitizing and glucose-lowering activity comparable to that of TZDs, and causes no significant increase in body weight or adipose tissue weight in db/db mice. In diabetic db/db mice, C333H elevated circulating high molecular weight adiponectin isoforms, decreased PPARγ 273 serine phosphorylation in brown adipose tissue and selectively modulated the expression of a subset of PPARγ target genes in adipose tissue. In vitro, C333H weakly recruited coactivator and weakly dissociated corepressor activity. These findings suggest that C333H has similar properties to SPPARMs and may be a potential therapeutic agent for the treatment of type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / drug effects*
  • Adipose Tissue, Brown / growth & development
  • Adipose Tissue, Brown / metabolism
  • Adipose Tissue, White / drug effects
  • Adipose Tissue, White / metabolism
  • Animals
  • Blood Glucose / analysis
  • Cell Line, Tumor
  • Diabetes Mellitus / metabolism*
  • Furans / pharmacology*
  • Gene Expression Regulation / drug effects
  • Humans
  • Insulin / blood
  • Insulin Resistance / physiology*
  • Leptin / blood
  • Male
  • Mice
  • Obesity / blood
  • Obesity / chemically induced
  • Organ Size / drug effects
  • Oxazoles / pharmacology*
  • PPAR gamma / agonists*
  • PPAR gamma / metabolism
  • Rats
  • Rats, Wistar
  • Sodium Glutamate

Substances

  • Blood Glucose
  • C333H compound
  • Furans
  • Insulin
  • Leptin
  • Oxazoles
  • PPAR gamma
  • Sodium Glutamate