Evaluation of four biodegradable, injectable bone cements in an experimental drill hole model in sheep

Eur J Pharm Biopharm. 2013 Sep;85(1):130-8. doi: 10.1016/j.ejpb.2013.04.013. Epub 2013 May 13.

Abstract

Four cement applications were tested in this investigation. Two dicalcium phosphate dihydrate (DCPD-brushite) hydraulic cements, an apatite hydraulic fiber loaded cement, and a calcium sulfate cement (Plaster of Paris) were implanted in epiphyseal and metaphyseal cylindrical bone defects in sheep. The in vivo study was performed to assess the biocompatibility and bone remodeling of four cement formulations. After time periods of 2, 4, and 6 months, the cement samples were clinically and histologically evaluated. Histomorphometrically, the amount of new bone formation, fibrous tissue, and bone marrow and the area of remaining cement were measured. In all specimens, no signs of inflammation were detectable either macroscopically or microscopically. Cements differed mainly in their resorption time. Calcium sulfate was already completely resorbed at 2 months and showed a variable amount of new bone formation and/or fibrous tissue in the original drill hole over all time periods. The two DCPD cements in contrast were degraded to a large amount at 6 months, whereas the apatite was almost unchanged over all time periods.

Keywords: Bone cements; Bone substitute; Calcium phosphate cements; Injectable cements; Synthetic cements.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Animals
  • Animals, Inbred Strains
  • Biocompatible Materials* / adverse effects
  • Bone Cements / adverse effects
  • Bone Cements / chemistry
  • Bone Cements / therapeutic use*
  • Bone Regeneration*
  • Bone Remodeling*
  • Bone and Bones / cytology
  • Bone and Bones / injuries
  • Bone and Bones / physiology
  • Bone and Bones / surgery*
  • Calcium Phosphates / administration & dosage
  • Calcium Phosphates / adverse effects
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / therapeutic use
  • Calcium Sulfate / administration & dosage
  • Calcium Sulfate / adverse effects
  • Calcium Sulfate / chemistry
  • Calcium Sulfate / therapeutic use
  • Epiphyses / injuries
  • Epiphyses / surgery
  • Female
  • Foreign-Body Reaction / prevention & control
  • Injections, Intralesional
  • Sheep, Domestic
  • Solubility
  • Time Factors
  • Viscosity

Substances

  • Biocompatible Materials
  • Bone Cements
  • Calcium Phosphates
  • Norian SRS Cement
  • beta-tricalcium phosphate
  • calcium phosphate, dibasic, dihydrate
  • Calcium Sulfate