Improvement of brain uptake for in vivo PET imaging of astrocytic oxidative metabolism using benzyl [1-(11)C]acetate

Appl Radiat Isot. 2013 Aug:78:102-7. doi: 10.1016/j.apradiso.2013.04.025. Epub 2013 Apr 25.

Abstract

Brain uptake of acetate is insufficient for obtaining a quantitative image of astrocytic oxidative metabolism. To improve the brain uptake of [1-(11)C]acetate, we synthesized benzyl [1-(11)C]acetate ([1-(11)C]BA) and conducted a positron emission tomography (PET) study assessing astrocytic oxidative metabolism. The brain uptake of [1-(11)C]BA was markedly higher compared with [1-(11)C]acetate, and disappeared with a half-life of 20 min in all regions studied. The brain uptake of [1-(11)C]BA was significantly decreased by fluorocitrate. The results indicate that [1-(11)C]BA could be a useful PET probe for assessing astrocytic oxidative metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / pharmacokinetics*
  • Animals
  • Astrocytes / diagnostic imaging*
  • Astrocytes / metabolism*
  • Brain / diagnostic imaging*
  • Brain / metabolism*
  • Carbon / pharmacokinetics*
  • Male
  • Metabolic Clearance Rate
  • Positron-Emission Tomography / methods*
  • Radiopharmaceuticals / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tissue Distribution

Substances

  • Acetates
  • Radiopharmaceuticals
  • carbon-11 acetate
  • Carbon