Inhibitory constituents of the heartwood of Dalbergia odorifera on nitric oxide production in RAW 264.7 macrophages

Bioorg Med Chem Lett. 2013 Jul 15;23(14):4263-6. doi: 10.1016/j.bmcl.2013.04.032. Epub 2013 Apr 24.

Abstract

Two new isoflavanones (1 and 13), along with 25 known compounds (2-12, 14-27), were isolated from the EtOAc-soluble fraction of the heartwood of Dalbergia odorifera by following their potential to inhibit the LPS-induced nitric oxide production in RAW 264.7 cells. The structures of the isolated compounds were established by spectroscopic data such as (1)D, (2)D NMR and MS spectrometry. Among the isolated compounds, (2S)-pinocembrin (26), showed the most potent inhibitory effect with IC50 value of 18.1μM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Dalbergia / chemistry*
  • Isoflavones / chemistry*
  • Isoflavones / isolation & purification
  • Isoflavones / pharmacology
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Mice
  • Molecular Conformation
  • Nitric Oxide / metabolism*
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology

Substances

  • Isoflavones
  • Lipopolysaccharides
  • Plant Extracts
  • Nitric Oxide