The effect of peroxisome proliferator-activated receptor-γ ligands on in vitro and in vivo models of COPD

Eur Respir J. 2014 Feb;43(2):409-20. doi: 10.1183/09031936.00187812. Epub 2013 Jun 21.

Abstract

Peroxisome proliferator-activated receptor (PPAR)-γ is expressed in alveolar macrophages. The anti-inflammatory potential of the PPAR-γ ligands rosiglitazone and pioglitazone were investigated using in vitro alveolar macrophage models and in vivo animal models relevant to chronic obstructive pulmonary disease (COPD). PPAR-γ protein and gene expression in COPD alveolar macrophages was compared with control smokers and never-smokers. COPD macrophages were used to investigate the effects of PPAR-γ ligands and corticosteroids on lipopolysaccharide-induced cytokine production, alternative macrophage activation (M2) gene expression and efferocytosis. The effects of PPAR-γ ligands in a subchronic tobacco smoke model in mice were investigated. PPAR-γ protein expression was similar in COPD patients compared to controls, although increased gene expression levels were observed in COPD patients and control smokers compared to never-smokers. PPAR-γ ligands reduced tumour necrosis factor-α and CC chemokine ligand-5, but not CXC chemokine ligand-8, in COPD alveolar macrophages; these effects were generally less than those of the corticosteroid dexamethasone. Rosiglitazone increased M2 gene expression and enhanced efferocytosis of apoptotic neutrophils. Rosiglitazone and pioglitazone attenuated airway neutrophilia in a corticosteroid-resistant mouse model of pulmonary inflammation. We show biological actions of PPAR-γ agonists on corticosteroid-resistant disease, tobacco smoke-induced pulmonary inflammation, skewing of macrophage phenotype and clearance of apoptotic neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Hormones / chemistry
  • Aged
  • Animals
  • Apoptosis
  • Chemokine CCL5 / metabolism
  • Dexamethasone / blood
  • Female
  • Gene Expression Regulation
  • Humans
  • Hypoglycemic Agents / administration & dosage
  • Inflammation
  • Interleukin-8 / metabolism
  • Ligands
  • Lipopolysaccharides / chemistry
  • Macrophages / metabolism
  • Macrophages, Alveolar / drug effects*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Middle Aged
  • Neutrophils / metabolism
  • PPAR gamma / metabolism*
  • Pioglitazone
  • Pulmonary Disease, Chronic Obstructive / drug therapy*
  • Pulmonary Disease, Chronic Obstructive / physiopathology*
  • Rosiglitazone
  • Smoking / adverse effects
  • Thiazolidinediones / administration & dosage
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Adrenal Cortex Hormones
  • CCL5 protein, human
  • Ccl5 protein, mouse
  • Chemokine CCL5
  • Hypoglycemic Agents
  • Interleukin-8
  • Ligands
  • Lipopolysaccharides
  • PPAR gamma
  • Thiazolidinediones
  • Tumor Necrosis Factor-alpha
  • Rosiglitazone
  • Dexamethasone
  • Pioglitazone