Comparison of vascular growth factors in the murine brain reveals placenta growth factor as prime candidate for CNS revascularization

Blood. 2013 Aug 1;122(5):658-65. doi: 10.1182/blood-2012-07-441527. Epub 2013 Jun 26.

Abstract

Vascular bypass procedures in the central nervous system (CNS) remain technically challenging, hindered by complications and often failing to prevent adverse outcome such as stroke. Thus, there is an unmet clinical need for a safe and effective CNS revascularization. Vascular endothelial growth factors (VEGFs) are promising candidates for revascularization; however, their effects appear to be tissue-specific and their potential in the CNS has not been fully explored. To test growth factors for angiogenesis in the CNS, we characterized the effects of endothelium-specific growth factors on the brain vasculature and parenchyma. Recombinant adeno-associated virus (AAV) vectors encoding the growth factors were injected transcranially to the frontoparietal cerebrum of mice. Angiogenesis, mural cell investment, leukocyte recruitment, vascular permeability, reactive gliosis and neuronal patterning were evaluated by 3-dimensional immunofluorescence, electron microscopy, optical projection tomography, and magnetic resonance imaging. Placenta growth factor (PlGF) stimulated robust angiogenesis and arteriogenesis without significant side effects, whereas VEGF and VEGF-C incited growth of aberrant vessels, severe edema, and inflammation. VEGF-B, angiopoietin-1, angiopoietin-2, and a VEGF/angiopoietin-1 chimera had minimal effects on the brain vessels or parenchyma. Of the growth factors tested, PlGF emerged as the most efficient and safe angiogenic factor, hence making it a candidate for therapeutic CNS revascularization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessels / growth & development
  • Blood Vessels / metabolism
  • Central Nervous System / blood supply*
  • Central Nervous System Neoplasms / etiology
  • Central Nervous System Neoplasms / genetics
  • Cerebral Revascularization*
  • Encephalitis / etiology
  • Encephalitis / genetics
  • Female
  • Genetic Therapy / methods
  • Hemangioma / etiology
  • Hemangioma / genetics
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Neovascularization, Physiologic / genetics
  • Placenta Growth Factor
  • Pregnancy Proteins / genetics
  • Pregnancy Proteins / metabolism
  • Pregnancy Proteins / physiology*
  • Pregnancy Proteins / therapeutic use
  • Vascular Endothelial Growth Factor A / adverse effects
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor C / adverse effects
  • Vascular Endothelial Growth Factor C / genetics

Substances

  • Intercellular Signaling Peptides and Proteins
  • Pgf protein, mouse
  • Pregnancy Proteins
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor C
  • Placenta Growth Factor