Methods to study the proliferation and differentiation of cardiac side population (CSP) cells

Methods Mol Biol. 2013:1036:95-106. doi: 10.1007/978-1-62703-511-8_9.

Abstract

Investigation of cardiac progenitor cell proliferation and differentiation is essential for both the basic understanding of progenitor cell biology as well as the development of cellular therapeutics for tissue regeneration. Herein, we describe techniques used for the analysis of CSP cell proliferation, cell cycle status, and cardiomyogenic differentiation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Cycle
  • Cell Differentiation*
  • Cell Proliferation*
  • Cell Separation / methods*
  • Cells, Cultured
  • Flow Cytometry
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism
  • Rats
  • Side-Population Cells / cytology*
  • Side-Population Cells / metabolism
  • Stem Cells / cytology*
  • Stem Cells / metabolism