Bioinert membranes prepared from amphiphilic poly(vinyl chloride)-g-poly(oxyethylene methacrylate) graft copolymers

Abstract

Poly(vinyl chloride) (PVC) membrane was hydrophilically modified by grafting with poly(oxyethylene methacrylate) (POEM) using atom transfer radical polymerization (ATRP). The successful grafting of PVC main chain by POEM was characterized by Fourier transform infrared spectroscopy (FT-IR). The molecular weight and hydrophilicity of membranes increased with the amount of POEM grafting, as characterized by gel permeation chromatography (GPC) and contact angle measurement, respectively. Transmission electron microscope (TEM) and small angle X-ray scattering (SAXS) analysis revealed the microphase-separated structure of PVC-g-POEM and the domain spacing increased from 59.3 to 86.1 nm with increasing grafting degree. Scanning electron microscopy (SEM) was used for the direct visualization of the mouse embryonic fibroblast (MEF) cell and bacteria adhesion on the membrane surface. Protein adsorption and eukaryotic and prokaryotic cell adhesion tests showed that the bioinert properties of membranes were significantly increased with POEM content.

Keywords: ATRP; Atom transfer radical polymerization; Cell adhesion; FT-IR; Fourier transform infrared spectroscopy; GPC; Gel permeation chromatography; Graft copolymer; MEF; Membranes; Mouse embryonic fibroblast; POEM; PVC; PVC-g-POEM; PVC:POEM=1:1; PVC:POEM=1:3; PVC:POEM=1:6; PVPO11; PVPO13; PVPO16; Poly(oxyethylene methacrylate); Poly(vinyl chloride); Poly(vinyl chloride)-g-poly(oxyethylenemethacrylate); Protein adsorption; SAXS; SEM; Scanning electron microscopy; Small angle X-ray scattering; TEM; Transmission electron microscopy.