Translational repression using BIV Tat peptide-TAR RNA interaction in mammalian cells

Chem Commun (Camb). 2013 Aug 28;49(67):7457-9. doi: 10.1039/c3cc43086c.

Abstract

We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5'-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expression of a ligand - the BIV Tat peptide.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 5' Untranslated Regions
  • Animals
  • Cell Line
  • Gene Expression Regulation
  • Immunodeficiency Virus, Bovine / genetics*
  • Peptides / genetics*
  • Protein Biosynthesis*
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics*
  • RNA, Viral / genetics
  • Viral Proteins / genetics*

Substances

  • 5' Untranslated Regions
  • Peptides
  • RNA, Messenger
  • RNA, Viral
  • Viral Proteins