Abstract
Background:
To study the expression of Syk (spleen tyrosine kinase) gene in human ESCC (esophageal squamous cell carcinoma).
Methods:
We used immunohistochemical staining to detect Syk protein expression in esophageal carcinoma tissues and RT-PCR (semi-quantitative reverse transcription PCR), Real-time PCR (Real-time quantitative PCR) and western blotting technique to detect the expression of Syk mRNA and protein in specimens from 4 esophageal cell lines.
Results:
Immunohistochemistry analyses showed that in the esophageal cancer tissues Syk protein was expressed inferior to the adjacent normal one, p < 0.05. Real-time PCR and Western blotting showed that low expression of the Syk gene was detected in the 4 esophageal cancer cell lines compared with positive and negative cell lines, p < 0.05.
Conclusions:
The low expression of the Syk gene may play an important role in tumorigenesis in esophageal cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / biosynthesis*
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Biomarkers, Tumor / genetics
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Blotting, Western
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Carcinoma, Squamous Cell / enzymology*
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Carcinoma, Squamous Cell / genetics
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Cell Line, Tumor
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism
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Esophageal Neoplasms / enzymology*
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Esophageal Neoplasms / genetics
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Esophageal Squamous Cell Carcinoma
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Genes, Tumor Suppressor*
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Humans
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Immunohistochemistry
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Intracellular Signaling Peptides and Proteins / biosynthesis*
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism
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Protein-Tyrosine Kinases / biosynthesis*
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Syk Kinase
Substances
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Biomarkers, Tumor
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Intracellular Signaling Peptides and Proteins
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RNA, Messenger
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Protein-Tyrosine Kinases
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SYK protein, human
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Syk Kinase