Inversion of enantioselectivity of a mononuclear non-heme iron(II)-dependent hydroxylase by tuning the interplay of metal-center geometry and protein structure

Angew Chem Int Ed Engl. 2013 Sep 9;52(37):9677-81. doi: 10.1002/anie.201304633. Epub 2013 Jul 23.

Authors

Sarah M Pratter¹, Cornelia Konstantinovics, Cristiana M L Di Giuro, Erich Leitner, Devesh Kumar, Sam P de Visser, Gideon Grogan, Grit D Straganz

Affiliation

¹ Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, 8010 Graz, Austria.

PMID: 23881738
DOI: 10.1002/anie.201304633

No abstract available

Keywords: asymmetric catalysis; hydroxylation; metalloenzymes; non-heme iron; protein design.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis
Crystallography, X-Ray
Hydroxylation
Iron / chemistry*
Kinetics
Mixed Function Oxygenases / chemistry*
Mixed Function Oxygenases / metabolism
Protein Structure, Tertiary
Stereoisomerism

Substances

Iron
Mixed Function Oxygenases