Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots

Melanie Krebs; Karin Schumacher

doi:10.1101/pdb.prot073031

Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots

Cold Spring Harb Protoc. 2013 Aug 1;2013(8):776-80. doi: 10.1101/pdb.prot073031.

Authors

Melanie Krebs¹, Karin Schumacher

Affiliation

¹ Department of Developmental Biology, Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany. melanie.krebs@cos.uni-heidelberg.de

PMID: 23906910
DOI: 10.1101/pdb.prot073031

Abstract

Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / cytology
Calcium / analysis*
Calcium-Binding Proteins / genetics
Calcium-Binding Proteins / metabolism*
Cations, Divalent / analysis
Cell Nucleus / chemistry*
Cytological Techniques / methods*
Cytoplasm / chemistry*
Fluorescent Dyes / metabolism*
Microscopy, Confocal / methods*
Plant Roots / cytology
Staining and Labeling / methods
Transgenes

Substances

Calcium-Binding Proteins
Cations, Divalent
Fluorescent Dyes
yellow cameleon
Calcium