Markers of oxidative stress and erythrocyte antioxidant enzyme activity in older men and women with differing physical activity

Exp Gerontol. 2013 Nov;48(11):1141-6. doi: 10.1016/j.exger.2013.07.010. Epub 2013 Jul 30.

Abstract

The aim of the present study was to examine the relationship between markers of oxidative stress and erythrocyte antioxidant enzyme activity and physical activity in older men and women. The present study included 481 participants (233 men and 248 women) in the age group 65-69 years (127 men and 125 women) and in the age group 90 years and over (106 men and 123 women). The classification of respondents by physical activity was based on answers to the question if, in the past 12 months, they engaged in any pastimes which require physical activity. The systemic oxidative stress status was assessed by measuring plasma iso-PGF2α and protein carbonyl concentration as well as erythrocyte antioxidant enzymes activity, i.e., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). The concentration of plasma iso-PGF2α and protein carbonyls (CP) was lower in groups of younger men and women compared to the respective older groups. In all examined groups, physical activity resulted in decrease of these oxidative stress markers and simultaneously caused adaptive increase in the erythrocyte SOD activity. Additionally, in active younger men CAT, GPx, and GR activities were higher than in sedentary ones. In conclusion, oxidative stress increase is age-related, but physical activity can reduce oxidative stress markers and induce adaptive increase in the erythrocyte antioxidant enzyme activity, especially SOD, even in old and very old men and women.

Keywords: Antioxidant enzymes; Elderly; Men and woman; Oxidative stress markers; Physical activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging / blood
  • Aging / metabolism
  • Aging / physiology*
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Catalase / blood
  • Dinoprost / analogs & derivatives
  • Dinoprost / blood
  • Erythrocytes / enzymology*
  • Female
  • Glutathione Peroxidase / blood
  • Glutathione Reductase / blood
  • Humans
  • Male
  • Motor Activity / physiology*
  • Oxidative Stress*
  • Protein Carbonylation
  • Superoxide Dismutase / blood

Substances

  • Biomarkers
  • 8-epi-prostaglandin F2alpha
  • Dinoprost
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase