An improved protocol for electroporation in members of the genus Gordonia

Pooja Singh; Preeti Srivastava

doi:10.1016/j.mimet.2013.07.025

An improved protocol for electroporation in members of the genus Gordonia

J Microbiol Methods. 2013 Nov;95(2):114-6. doi: 10.1016/j.mimet.2013.07.025. Epub 2013 Aug 8.

Authors

Pooja Singh¹, Preeti Srivastava

Affiliation

¹ Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.

PMID: 23933494
DOI: 10.1016/j.mimet.2013.07.025

Abstract

Gordonia are high GC gram-positive bacteria that have not yet been exploited well for biotechnological purposes because of the limited genetic tools. Described here is an improved protocol for electroporation, which is useful for several Gordonia species. The maximum transformation efficiency obtained was 2.8 × 10(4)/μg (Gordonia rubropertinctus, Gordonia sp), and 1.7 × 10(3)/μg (Gordonia amarae).

Keywords: Gordonia; Plasmid pRSG43; Transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotechnology
Electroporation / methods*
Gordonia Bacterium / genetics*
Plasmids / genetics