Abstract
Gemella haemolysans is an uncommon but described cause of invasive disease in humans. We report a case of meningitis due to G. haemolysans that did not grow in cerebrospinal fluid culture, demonstrating a potential role for direct 16S rRNA gene PCR and sequencing in culture-negative cerebrospinal fluid when bacterial meningitis is suspected.
MeSH terms
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Aged
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Bacteriological Techniques
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Gemella / isolation & purification*
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Gram-Positive Bacterial Infections / diagnosis*
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Gram-Positive Bacterial Infections / microbiology*
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Gram-Positive Bacterial Infections / pathology
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Humans
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Male
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Meningoencephalitis / diagnosis*
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Meningoencephalitis / microbiology*
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Meningoencephalitis / pathology
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Microscopy
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S