Differential potential for envelope glycoprotein-mediated steric shielding of host cell surface proteins among filoviruses

Virology. 2013 Nov;446(1-2):152-61. doi: 10.1016/j.virol.2013.07.029. Epub 2013 Aug 28.

Abstract

The viral envelope glycoprotein (GP) is thought to play important roles in the pathogenesis of filovirus infection. It is known that GP expressed on the cell surface forms a steric shield over host proteins such as major histocompatibility complex class I and integrin β1, which may result in the disorder of cell-to-cell contacts and/or inhibition of the immune response. However, it is not clarified whether this phenomenon contributes to the pathogenicity of filoviruses. In this study, we found that the steric shielding efficiency differed among filovirus strains and was correlated with the difference in their relative pathogenicities. While the highly glycosylated mucin-like region of GP was indispensable, the differential shielding efficiency did not necessarily depend on the primary structure of the mucin-like region, suggesting the importance of the overall properties (e.g., flexibility and stability) of the GP molecule for efficient shielding of host proteins.

Keywords: Ebola virus; Glycoprotein; Integrin; MHC; Marburg virus; Pathogenicity; Steric shielding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • DNA Mutational Analysis
  • Filoviridae / pathogenicity*
  • Host-Pathogen Interactions*
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Protein Binding
  • Protein Interaction Mapping
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / metabolism*
  • Virulence

Substances

  • Membrane Proteins
  • Viral Envelope Proteins