TCam-2 seminoma cells exposed to egg-derived microenvironment modify their shape, adhesive pattern and migratory behaviour: a molecular and morphometric analysis

PLoS One. 2013 Oct 1;8(10):e76192. doi: 10.1371/journal.pone.0076192. eCollection 2013.

Abstract

Seminoma is one of the most common Testicular Germ Cell Tumours that originates during embryonic development due to an alteration of the local niche that in turn results in a delayed or blocked differentiation of Primordial Germ Cells. The block of differentiation is actually a common way to develop cancer disease as postulated by the "embryonic rest theory of cancer". In agreement with this theory different studies have demonstrated that embryonic cues display the capacity of reprogramming aggressive cancer cells towards a less aggressive phenotype. Herein we investigate the ability of a culture medium added with 10% egg albumen (EW, Egg White) to modulate seminoma cell phenotype and behaviour, by ensuring a proper set of morphogenetic signals. We chose to use the TCam-2 seminoma cell line that has been established as the only available cell line, obtained from a primary testicular seminoma. EW is able to: 1) modify TCam-2 cell spreading rate and cell-substrate adhesion without affecting proliferation and survival indexes; 2) modulate TCam-2 actin distribution pattern increasing cortical localization of actin filaments; 3) increase TCam-2 cell-cell junction capability; 4) decrease both chemo-sensitive and collective TCam-2 migratory behaviour. According to these observations morphometric fractal analysis revealed the ability of EW to increase Circularity and Solidity parameters and, consequently, to decrease Fractal dimension. Prompted by these observations we hypothesize that EW treatment could rescue, at least in part, the neoplastic-metastatic behaviour of seminoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Cadherins / metabolism
  • Cell Adhesion / drug effects
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects
  • Cell Survival / drug effects
  • Connexin 43 / metabolism
  • Egg Proteins / pharmacology*
  • Egg White
  • Focal Adhesions / drug effects
  • Humans
  • Integrin beta1 / metabolism
  • Intercellular Junctions / metabolism
  • Intercellular Junctions / ultrastructure
  • Male
  • Seminoma / genetics
  • Seminoma / metabolism*
  • Seminoma / pathology*
  • Testicular Neoplasms / genetics
  • Testicular Neoplasms / metabolism*
  • Testicular Neoplasms / pathology*
  • Vinculin / metabolism
  • beta Catenin / metabolism

Substances

  • Actins
  • Cadherins
  • Connexin 43
  • Egg Proteins
  • Integrin beta1
  • beta Catenin
  • Vinculin

Grants and funding

The present study was supported by PRIN 2008 - Angela Catizone (grant number 2008NE3XPK_002; http://sito.cineca.it/) and by Ateneo Federato 2009 – Angela Catizone (grant number C26F09AHLB; http://uniroma1.cineca.it). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.