A simple and rapid method of quantitative analysis of phosphoamino acids by high-performance liquid chromatography

Anal Biochem. 1985 Jul;148(1):207-12. doi: 10.1016/0003-2697(85)90647-5.

Abstract

A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue of this uv-active eluant. The technique is sensitive to 200 pmol of phosphoamino acid and has been shown to be directly applicable to the analysis of isolated phosphoproteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Phosphates / analysis
  • Phosphoproteins / analysis
  • Phosphorylation
  • Phosphoserine / analysis*
  • Phosphothreonine / analysis*
  • Phosphotyrosine
  • Serine / analogs & derivatives*
  • Threonine / analogs & derivatives*
  • Tyrosine / analogs & derivatives*
  • Tyrosine / analysis

Substances

  • Phosphates
  • Phosphoproteins
  • Phosphothreonine
  • Phosphoserine
  • Phosphotyrosine
  • Threonine
  • Tyrosine
  • Serine