Abstract
A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue of this uv-active eluant. The technique is sensitive to 200 pmol of phosphoamino acid and has been shown to be directly applicable to the analysis of isolated phosphoproteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid / methods
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Phosphates / analysis
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Phosphoproteins / analysis
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Phosphorylation
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Phosphoserine / analysis*
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Phosphothreonine / analysis*
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Phosphotyrosine
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Serine / analogs & derivatives*
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Threonine / analogs & derivatives*
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Tyrosine / analogs & derivatives*
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Tyrosine / analysis
Substances
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Phosphates
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Phosphoproteins
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Phosphothreonine
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Phosphoserine
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Phosphotyrosine
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Threonine
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Tyrosine
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Serine