The estimation of nitrous oxide emissions is complicated by the high degree of uncertainty on the emission factors involved and by the limited acquaintance with all significant nitrous oxide sources. A potentially important source for which emission data are lacking is the sewage system transporting waste water from human activities. For this study an experimental measurement campaign has been carried out on waste water sampled at different sewage treatment plants. The nitrous oxide developing from the water samples was monitored by means of gas chromatography. The methodological analysis was based on the concentration/time curves obtained. Our results indicate that the formation of nitrous oxide from the waste water matrices results from microbiological denitrification. We deduced tentative emission factors for the waste water types studied.