An animal model of MERS produced by infection of rhesus macaques with MERS coronavirus

J Infect Dis. 2014 Jan 15;209(2):236-42. doi: 10.1093/infdis/jit590. Epub 2013 Nov 11.

Abstract

In 2012, a novel coronavirus (CoV) associated with severe respiratory disease, Middle East respiratory syndrome (MERS-CoV; previously known as human coronavirus-Erasmus Medical Center or hCoV-EMC), emerged in the Arabian Peninsula. To date, 114 human cases of MERS-CoV have been reported, with 54 fatalities. Animal models for MERS-CoV infection of humans are needed to elucidate MERS pathogenesis and to develop vaccines and antivirals. In this study, we developed rhesus macaques as a model for MERS-CoV using intratracheal inoculation. The infected monkeys showed clinical signs of disease, virus replication, histological lesions, and neutralizing antibody production, indicating that this monkey model is suitable for studies of MERS-CoV infection.

Keywords: MERS; animal model; coronavirus; pathology; rhesus monkey.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood
  • Coronavirus / physiology*
  • Coronavirus Infections / pathology*
  • Coronavirus Infections / virology*
  • Disease Models, Animal
  • Histocytochemistry
  • Macaca mulatta
  • Virus Replication

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral