Urine biomarkers for monitoring juvenile lupus nephritis: a prospective longitudinal study

Pediatr Nephrol. 2014 Mar;29(3):397-405. doi: 10.1007/s00467-013-2668-4. Epub 2013 Nov 16.

Abstract

Background: In juvenile-onset systemic lupus erythematosus (JSLE), renal involvement (lupus nephritis) is frequently seen and can result in long-term morbidity. This prospective longitudinal study aimed to identify the utility of standard and/or novel biomarkers for monitoring and predicting lupus nephritis in a real world setting.

Methods: Using an unselected JSLE cohort, urine samples were collected during routine clinical review. Protein concentrations of urinary monocyte chemo-attractant protein 1 (uMCP1) and neutrophil gelatinase-associated lipocalin (uNGAL) were analysed along with standard disease activity markers, and were compared with current and subsequent disease activity.

Results: JSLE patients (n = 64; median age 14.1 years) were seen at 3 (interquartile range: 2-5) clinical reviews over 364 (182-532) days. Multivariate analysis demonstrated uMCP1 and serum C3 as independent variables (p < 0.001) for active renal disease at the time of the current review. uMCP1 was an excellent predictor of improved renal disease over time (AUC: 0.81; p = 0.013). uNGAL was a good predictor of worsened renal disease activity (AUC 0.76; p = 0.04) over time.

Conclusion: Biomarkers (uMCP1, serum C3) can indicate current renal involvement in JSLE, whilst uMCP1 and uNGAL are able to predict subsequent renal disease activity changes. Moving towards biomarker-led monitoring may improve the renal outcome for our patients.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / urine*
  • Adolescent
  • Age of Onset
  • Biomarkers / blood
  • Biomarkers / urine
  • Case-Control Studies
  • Chemokine CCL2 / urine*
  • Chi-Square Distribution
  • Child
  • Child, Preschool
  • Complement C3 / metabolism
  • Disease Progression
  • Female
  • Humans
  • Infant
  • Linear Models
  • Lipocalin-2
  • Lipocalins / urine*
  • Longitudinal Studies
  • Lupus Nephritis / blood
  • Lupus Nephritis / diagnosis*
  • Lupus Nephritis / therapy
  • Lupus Nephritis / urine
  • Male
  • Multivariate Analysis
  • Predictive Value of Tests
  • Prognosis
  • Prospective Studies
  • Proto-Oncogene Proteins / urine*
  • Time Factors
  • United Kingdom
  • Urinalysis

Substances

  • Acute-Phase Proteins
  • Biomarkers
  • C3 protein, human
  • CCL2 protein, human
  • Chemokine CCL2
  • Complement C3
  • LCN2 protein, human
  • Lipocalin-2
  • Lipocalins
  • Proto-Oncogene Proteins