Highly specific enrichment of N-linked glycopeptides based on hydrazide functionalized soluble nanopolymers

Chem Commun (Camb). 2014 Jan 28;50(8):1027-9. doi: 10.1039/c3cc47347c.

Abstract

In this work, for the first time, hydrazide functionalized PAMAM was designed and synthesized for efficient and selective enrichment of N-linked glycopeptides from complex biological samples using FASP (filter-aided sample preparation) mode.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Dendrimers / chemistry*
  • Glycopeptides / chemical synthesis
  • Glycopeptides / chemistry*
  • Glycopeptides / metabolism
  • Glycosylation
  • Molecular Sequence Data
  • Nanostructures / chemistry
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism
  • Polymers / chemistry*
  • Solubility
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Dendrimers
  • Glycopeptides
  • PAMAM Starburst
  • Polymers
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase