BSA-boronic acid conjugate as lectin mimetics

Biochem Biophys Res Commun. 2014 Jan 10;443(2):562-7. doi: 10.1016/j.bbrc.2013.12.006. Epub 2013 Dec 8.

Abstract

We report bovine serum albumin (BSA)-boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA-BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS-PAGE gel. The BSA-BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA-BA conjugates was conducted by immobilizing BSA-BA onto SPR gold chip. Overall, we demonstrated a BSA-BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.

Keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide; ARS; Alizarin Red S; BA; BSA; Boronic acid; Bovine serum albumin; Carbohydrate; EDC; Lectin; SPR; Surface plasmon resonance; boronic acid; bovine serum albumin; surface plasmon resonance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomimetic Materials / chemical synthesis*
  • Biosensing Techniques / methods*
  • Boronic Acids / chemistry*
  • Glycomics / methods
  • Lectins / chemistry*
  • Polysaccharides / analysis*
  • Polysaccharides / chemistry
  • Serum Albumin, Bovine / chemistry*
  • Surface Plasmon Resonance / methods*

Substances

  • Boronic Acids
  • Lectins
  • Polysaccharides
  • Serum Albumin, Bovine