Imaging live cell in micro-liquid enclosure by X-ray laser diffraction

Nat Commun. 2014:5:3052. doi: 10.1038/ncomms4052.

Abstract

Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation damage processes. In bioimaging, it is essential to keep the sample close to its natural state. Conventional high-resolution imaging, however, suffers from severe radiation damage that hinders live cell imaging. Here we present a method for capturing snapshots of live cells kept in a micro-liquid enclosure array by X-ray laser diffraction. We place living Microbacterium lacticum cells in an enclosure array and successively expose each enclosure to a single X-ray laser pulse from the SPring-8 Angstrom Compact Free-Electron Laser. The enclosure itself works as a guard slit and allows us to record a coherent diffraction pattern from a weakly-scattering submicrometre-sized cell with a clear fringe extending up to a 28-nm full-period resolution. The reconstructed image reveals living whole-cell structures without any staining, which helps advance understanding of intracellular phenomena.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / cytology*
  • Animals
  • Imaging, Three-Dimensional / methods*
  • Lasers
  • Scattering, Radiation
  • X-Ray Diffraction / methods*