IL-15 deficient tax mice reveal a role for IL-1α in tumor immunity

PLoS One. 2014 Jan 8;9(1):e85028. doi: 10.1371/journal.pone.0085028. eCollection 2014.

Abstract

IL-15 is recognized as a promising candidate for tumor immunotherapy and has been described as both a promoter of cancer and a promoter of anti-cancer immunity. IL-15 was discovered in cells transformed by HTLV-1, the etiologic agent of adult T cell leukemia/lymphoma (ATL) and the human retrovirus that carries the Tax oncogene. We have developed the TAX-LUC mouse model of ATL in which Tax expression drives both malignant transformation and luciferase expression, enabling non-invasive imaging of tumorigenesis in real time. To identify the role of IL-15 in spontaneous development of lymphoma in vivo, an IL-15(-/-) TAX-LUC strain was developed and examined. The absence of IL-15 resulted in aggressive tumor growth and accelerated mortality and demonstrated that IL-15 was not required for Tax-mediated lymphoma but was essential for anti-tumor immunity. Further analysis revealed a unique transcriptional profile in tumor cells that arise in the absence of IL-15 that included a significant increase in the expression of IL-1α and IL-1α-regulated cytokines. Moreover, anti-IL-1α antibodies and an IL-1 receptor antagonist (Anakinra) were used to interrogate the potential of IL-1α targeted therapies in this model. Taken together, these findings identify IL-15 and IL-1α as therapeutic targets in lymphoma.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Disease Models, Animal
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / immunology*
  • Gene Products, tax / genetics
  • Gene Products, tax / immunology
  • Genes, Reporter
  • HTLV-I Infections / genetics
  • HTLV-I Infections / immunology*
  • HTLV-I Infections / pathology
  • HTLV-I Infections / virology
  • Human T-lymphotropic virus 1 / immunology
  • Immunity, Innate*
  • Interleukin 1 Receptor Antagonist Protein / pharmacology
  • Interleukin-15 / deficiency
  • Interleukin-15 / genetics
  • Interleukin-15 / immunology*
  • Interleukin-1alpha / antagonists & inhibitors
  • Interleukin-1alpha / genetics
  • Interleukin-1alpha / immunology*
  • Leukemia-Lymphoma, Adult T-Cell / genetics
  • Leukemia-Lymphoma, Adult T-Cell / immunology*
  • Leukemia-Lymphoma, Adult T-Cell / pathology
  • Leukemia-Lymphoma, Adult T-Cell / virology
  • Luciferases / genetics
  • Luciferases / immunology
  • Mice
  • Molecular Imaging
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Tumor Burden
  • Tumor Cells, Cultured

Substances

  • Gene Products, tax
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-15
  • Interleukin-1alpha
  • tax protein, Human T-lymphotrophic virus 1
  • Luciferases