Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers

Abstract

Four trichothecenes (Fus-X, 3ADON, DAS and T-2) were detected in potato tubers inoculated with Fusarium spp. by UPLC-MS/MS. The influence of cultivars, Fusarium strains and storage temperature on trichothecenes production was evaluated. The concentration of trichothecenecs was much higher in susceptible cultivar (Longshu No. 3) than in resistant one (Longshu No. 6). The susceptible cultivar infected with Fusarium sulphureum had the maximum concentration of Fus-X, 3ADON and DAS. Among the three Fusarium strains, Fusarium solani had the strongest ability to produce T-2 in both susceptible and resistant cultivars. Room temperature storage was more likely to accumulate trichothecenes than low temperature storage. Meanwhile, the trichothecenes were found not only in the lesion but also in the adjacent asymptomatic tissue. Trichothecenes concentration showed a strong trend of decline with increase in distance from the infection point.

Keywords: 15-acetyldeoxynivalenol; 15ADON; 3-acetyldeoxynivalenol; 3ADON; DAS; DON; Distribution; Fus-X; Fusarenon X; Fusarium spp.; Mycotoxins; NEO; Potato tubers; ROS; T-2; T-2 Toxin; UPLC–MS/MS; deoxynivalenol; diacetoxyscirpenol; neosolaniol; reactive oxygen species; ultra-high performance liquid chromatography coupled tandem mass spectrometry.