Association study of human leukocyte antigen-DRB1 alleles with rheumatoid arthritis in Algerian patients

Int J Rheum Dis. 2017 Dec;20(12):1993-1997. doi: 10.1111/1756-185X.12272. Epub 2014 Jan 22.

Abstract

Introduction: Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory and multifactorial disease. Genetic predisposition seems to play an important role. The aim of this study is to explore the relationship between human leukocyte antigen (HLA)-DRB1 alleles and susceptibility, clinical and biological features of RA in an Algerian patient population.

Methods: Using polymerase chain reaction - sequence specific primers (SSP), 134 RA patients and 132 healthy controls were genotyped for HLA-DRB1 and HLA-DRB1*04 subtypes.

Results: HLA-DRB1*04 was found to have increased frequency in the RA group compared to controls (P < 0.001, OR = 3.14), and was associated with anti-citrullinated protein antibodies positivity (ACPA) (P = 0.01, OR = 2.35). In contrast, HLA-DRB1*07 was found to have a decreased frequency in patients compared to controls (P = 0.003, OR = 0.44) and significant decrease was observed in patients with the rheumatoid factor (RF) positivity subgroup (P = 0.009, OR = 0.29). HLA-DRB1*04:05 was associated with RA (P = 0.005, OR = 3.41), whereas, HLA-DRB1*04:02 showed a protective effect against RA (P = 0.003, OR = 0.20).

Conclusions: HLA-DRB1*04 was associated with increased risk for RA and ACPA positivity, while HLA-DRB1*07 was associated with reduced risk for RA and RF synthesis in Algerian patients.

Keywords: HLA-DRB1 alleles; anti-cyclic peptides antibodies; rheumatoid arthritis; rheumatoid factor.

Publication types

  • Multicenter Study

MeSH terms

  • Adult
  • Algeria
  • Anti-Citrullinated Protein Antibodies / blood
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / diagnosis
  • Arthritis, Rheumatoid / genetics*
  • Arthritis, Rheumatoid / immunology
  • Biomarkers / blood
  • Case-Control Studies
  • Chi-Square Distribution
  • Female
  • Gene Frequency
  • Genetic Association Studies
  • Genetic Markers
  • Genetic Predisposition to Disease
  • HLA-DRB1 Chains / genetics*
  • HLA-DRB1 Chains / immunology
  • Humans
  • Male
  • Middle Aged
  • Odds Ratio
  • Phenotype
  • Protective Factors
  • Rheumatoid Factor / blood
  • Risk Factors
  • Young Adult

Substances

  • Anti-Citrullinated Protein Antibodies
  • Biomarkers
  • Genetic Markers
  • HLA-DRB1 Chains
  • Rheumatoid Factor