The effects of Phα1β, a spider toxin, calcium channel blocker, in a mouse fibromyalgia model

Toxicon. 2014 Apr:81:37-42. doi: 10.1016/j.toxicon.2014.01.015. Epub 2014 Jan 31.

Abstract

This study investigated the effects of Phα1β, pregabalin and diclofenac using an animal model of fibromyalgia (FM). Repeated administration of reserpine (0.25 mg/kg sc) once daily for three consecutive days significantly decreased thermal hyperalgesia, mechanical allodynia, and dopamine and serotonin content in the brain on the 4th day. Phα1β and pregabalin treatment completely reverted the mechanical allodynia and thermal hyperalgesia induced by reserpine treatment on the 4th day, but diclofenac was ineffective. Reserpine treatment significantly increased the immobility time in the forced swim test, which is indicative of depression in the animals. Phα1β, but not pregabalin, reduced the immobility time (56%), suggesting that Phα1β may control persistent pathological pain in FM.

Keywords: Diclofenac; Fibromyalgia model; Phα1β; Pregabalin; Treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / metabolism
  • Calcium Channel Blockers / pharmacology
  • Calcium Channel Blockers / therapeutic use*
  • Diclofenac / pharmacology
  • Diclofenac / therapeutic use
  • Disease Models, Animal
  • Dopamine / metabolism
  • Fibromyalgia / drug therapy*
  • Hyperalgesia / chemically induced
  • Hyperalgesia / drug therapy*
  • Male
  • Mice
  • Pain / drug therapy
  • Pregabalin
  • Reserpine
  • Serotonin / metabolism
  • Spider Venoms / pharmacology
  • Spider Venoms / therapeutic use*
  • Spiders
  • gamma-Aminobutyric Acid / analogs & derivatives
  • gamma-Aminobutyric Acid / pharmacology
  • gamma-Aminobutyric Acid / therapeutic use

Substances

  • Calcium Channel Blockers
  • Spider Venoms
  • Diclofenac
  • Serotonin
  • Pregabalin
  • gamma-Aminobutyric Acid
  • Reserpine
  • Dopamine