Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice

Chuantian Xu; Hualan Chen; Beixia Hu; Shaohua Yang; Lin Zhang; Qinghua Huang; Xiumei Zhang

doi:10.1016/j.virusres.2013.10.002

Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice

Virus Res. 2013 Dec 26;178(2):511-516. doi: 10.1016/j.virusres.2013.10.002. Epub 2013 Oct 25.

Authors

Chuantian Xu¹, Hualan Chen², Beixia Hu¹, Shaohua Yang¹, Lin Zhang¹, Qinghua Huang¹, Xiumei Zhang³

Affiliations

¹ Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
² Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People's Republic of China.
³ Shandong Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China. Electronic address: xcttaian2002@163.com.

PMID: 24512754
DOI: 10.1016/j.virusres.2013.10.002

Abstract

A/swine/Fujian/1/2001 (FJ1) and A/Duck/Zhejiang/52/2000 (DK52) are H5N1 influenza viruses that are lethal in chickens. However, in mice, FJ1 is highly pathogenic, whereas DK52 cannot replicate at all. In this study, we used reverse genetics to demonstrate that amino acid residues at position 54 of polymerase acidic protein (PA) and position 26 of ion channel protein M2 of FJ1 and DK52 are important determinants for replication in mice. In addition, we prove that M2 and PA proteins contribute to the replication of H5N1 viruses in mice.

Keywords: M2; Mice; PA; Replication.