An epitope recognised by inhibitory monoclonal antibodies that react with a 51 kilodalton merozoite surface antigen in Plasmodium falciparum

Mol Biochem Parasitol. 1988 Feb;28(1):1-10. doi: 10.1016/0166-6851(88)90173-9.

Abstract

Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. These antibodies recognise a glycosylated antigen of approximately 51 kDa which is located on the merozoite surface membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Protozoan / genetics
  • Antigens, Protozoan / immunology*
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis
  • Epitopes / genetics
  • Epitopes / immunology
  • Erythrocytes / parasitology
  • Fluorescent Antibody Technique
  • Hybridomas
  • Immunoassay
  • Immunohistochemistry
  • Microscopy, Electron
  • Molecular Sequence Data
  • Plasmodium falciparum / genetics
  • Plasmodium falciparum / immunology*
  • Plasmodium falciparum / ultrastructure

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • Antigens, Surface
  • Epitopes
  • DNA

Associated data

  • GENBANK/M18825