Induction of human plasmablasts during infection with antibiotic-resistant nosocomial bacteria

J Antimicrob Chemother. 2014 Jul;69(7):1830-3. doi: 10.1093/jac/dku047. Epub 2014 Feb 28.

Abstract

Objectives: Nosocomial pathogens such as Acinetobacter baumannii are a growing public health threat, due in part to their increasing resistance to antibiotics. Since some strains are resistant to all available antibiotics, novel therapies are urgently needed. Plasmablasts are short-lived B cells found in the blood that can be collected and harnessed to produce therapeutic antibodies. We set out to determine whether plasmablasts are induced during infection with A. baumannii and other nosocomial pathogens.

Methods: We obtained blood samples from patients infected with antibiotic-resistant nosocomial pathogens, and analysed their plasmablast response by flow cytometry.

Results: We observed a strong induction of plasmablasts in patients with antibiotic-resistant A. baumannii infection. Furthermore, plasmablasts were also induced in response to other drug-resistant nosocomial pathogens.

Conclusions: These data suggest that plasmablasts may be broadly harnessed to develop therapeutic antibodies to combat otherwise untreatable antibiotic-resistant infections.

Keywords: Acinetobacter baumannii; antibody therapy; nosocomial infections.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / drug effects
  • Acinetobacter baumannii / immunology*
  • Acinetobacter baumannii / isolation & purification
  • Adult
  • Aged
  • Cross Infection / microbiology*
  • Drug Resistance, Bacterial
  • Female
  • Flow Cytometry
  • Humans
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Plasma Cells / immunology*