Abstract
A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.
Keywords:
KPC; Klebsiella pneumoniae carbapenemase; Pyrosequencing; Real-time PCR.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / classification*
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Bacterial Proteins / genetics*
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Genetic Variation*
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Humans
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Klebsiella Infections / epidemiology
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / classification
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics
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Molecular Epidemiology / methods
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Sequence Analysis, DNA / methods*
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beta-Lactamases / classification*
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beta-Lactamases / genetics*
Substances
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Bacterial Proteins
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beta-Lactamases
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carbapenemase