Overexpression of LAPTM4B-35: a novel marker of poor prognosis of prostate cancer

PLoS One. 2014 Mar 20;9(3):e91069. doi: 10.1371/journal.pone.0091069. eCollection 2014.

Abstract

Background: Lysosome-associated protein transmembrane 4b-35 (LAPTM4B-35) is a member of the mammalian 4-tetratransmembrane spanning protein superfamily, which is overexpressed in several solid malignancies. However, the expression of LAPTM4B-35 and its role in the progression of prostate cancer (PCa) is unknown. The aim of the present study was to investigate the LAPTM4B-35 expression in PCa and its potential relevance to clinicopathological variables and prognosis.

Methods: Immunohistochemistry was used to determine the expression of LAPTM4B-35 protein in 180 PCa tissues in comparison with 180 normal benign prostatic hyperplasia (BPH) specimens. The correlation between the expression of the LAPTM4B-35 protein and the clinicopathologic characteristics of patients with PCa was analyzed.

Results: Statistical analysis showed that LAPTM4B-35 expression was significantly elevated in PCa compared with the BPH controls. High LAPTM4B-35 staining was present in 71.11% of all the cases with PCa. The overexpression of LAPTM4B-35 was significantly associated with the lymph node metastasis, seminal vesicle invasion, PCa stage, higher Gleason score, higher preoperative PSA, and biochemical recurrence (BCR). The Kaplan-Meier survival analysis showed that the high expression of LAPTM4B-35 was related to the poor overall survival and BCR-free survival of patients with PCa. Multivariate Cox analysis showed that LAPTM4B-35 was an independent prognostic factor for both overall survival and BCR-free survival of patients with PCa.

Conclusions: Overexpression of LAPTM4B-35 may be associated with tumor progression and poor prognosis in PCa and thus may serve as a new molecular marker to predict the prognosis of PCa patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers, Tumor / metabolism*
  • Disease-Free Survival
  • Humans
  • Male
  • Membrane Proteins / metabolism*
  • Multivariate Analysis
  • Neoplasm Recurrence, Local / pathology
  • Oncogene Proteins / metabolism*
  • Proportional Hazards Models
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • LAPTM4B protein, human
  • Membrane Proteins
  • Oncogene Proteins

Grants and funding

This study was supported by the National Natural Science Foundation of China (NO: 81172451), Tianjin Major Anti-Cancer Project (12ZCDZSY17200), and Science Foundation of Tianjin medical university. (NO: 2009GSI18). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.