Abstract
The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Mice
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Oncogene Proteins v-abl / analysis*
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Oncogene Proteins, Fusion / analysis*
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Protein Array Analysis / methods*
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Proto-Oncogene Proteins c-bcr / analysis*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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Oncogene Proteins v-abl
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Oncogene Proteins, Fusion
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Proto-Oncogene Proteins c-bcr